PRALINE sequence alignment results for job Example_8

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The PRALINE alignment process was completed in 1600.0 seconds. Alignment score = 136062.00 Alignment score per aligned residue pair = 12.07 Sequence identities = 4822 Percent sequence identity = 0.43 Number of sequences = 14 Alignment length = 185 Number of residues = 1916 Number of gaps = 674

Download the final alignment [msl_ref7.msf] Download PRALINE raw output file [results.out] Download PSI-BLAST output files Download Transmembrane structure prediction files

Results colour-coded for amino acid conservation The current colourscheme of the alignment is for amino acid conservation.

The conservation scoring is performed by PRALINE. The scoring scheme works from 0 for the least conserved alignment position, up to 10 for the most conserved alignment position. The colour assignments are: Unconserved 0 1 2 3 4 5 6 7 8 9 10 Conserved   . . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 MSCL_ECOLI - - - - - - - - - M S I I K E F R E F A M R G N V V D L A V G V I I G A A F G K I V S S L V A D I I MSCL_ERWCA - - - - - - - - - M S I I K E F R E F A M R G N V V D L A V G V I I G A L F G K I V S S L V S D I I MSCL_PSEFL - - - - - - - - - M G L L S E F K A F A V K G N V V D M A V G I I I G A A F G K I V S S F V G D V I MSCL_CLOHI - - - - - - - - - - - M W K D F K E F A M K G N V V D L A V G V I I G G A F G K I V T S L V N D V I MSCL_BACSU - - - - - - - - - - - M W N E F K A F A M R G N I V D L A I G V V I G G A F G K I V T S L V N D I I MSCL_Q9RH75 - M S G V D E K G R R M L K E F R E F A M K G N V V D L A V G V I I R R A F G A I V T S L V G D V I MSCL_HAEIN - - - - - - - - - M N F I K E F R E F A M R G N V V D M A V G V I I G S A F G K I V S S L V S D I F MSCL_SYNY3 M V K S A R Q G A G G F W R D F K D F I L R G N V V D L A V A V V I G G A F T S I V N A F V A W L M MSCL_Q9RRR4 - - - - - - - - - - - M L N G F R D F I L R G N V V D L A V G V V I G A A F N N V V A A F T K A F L MSCL_CLOPE - - - - - - - - - - - M W K E F K E F A M K G N V I D L A I G V V I G G A F G K I V T S L V N D I I MSCL_MYCTU - - - - - - - - - - - M L K G F K E F L A R G N I V D L A V A V V I G T A F T A L V T K F T D S I I MSCL_MYCLE - - - - - - - - - - - M F R G F K E F L S R G N I V D L A V A V V I G T A F T A L I T K F T D S I I MSCL_STAAU - - - - - - - - - - - M L K E F K E F A L K G N V L D L A I A V V M G A A F N K I I S S L V E N I I 1msla - - - - - - - - - - - - - - - - - - - - A R G N I V D L A V A V V I G T A F T A L V T K F T D S I I Consistency 0 0 0 0 0 0 0 0 0 0 0 6 4 6 5 8 7 6 8 5 6 8 * * 9 9 * 9 * 9 7 9 9 9 8 5 9 * 5 6 8 9 7 7 7 7 4 5 8 8     . . . . . . . . . 60 . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 MSCL_ECOLI M P P L G L L I G G I D F K Q F A V T L R D - - - - - - - A Q G D I P A V V M H Y G V F I Q N V F D MSCL_ERWCA M P P L G L L I G G V D F K Q F A L F L R N - - - - - - - A Q G G I P A V V M N Y G A F I Q N I F D MSCL_PSEFL M P P I G L L I G G V D F S D L A I T L K A - - - - - - - E - G D V P A V V L A Y R K F I Q T V L N MSCL_CLOHI M P I L G L I L G G I N F T S A K L T L H G - - - - - - - L N S E K P - L T L N Y G Q F I Q N I L D MSCL_BACSU M P L V G L L L G G L D F S G L S F T F G D - - - - - - - A - - - - - - - V V K Y G S F I Q T I V N MSCL_Q9RH75 M P L I G A V T G G L D F S N Y F T P L S K A V T A T N L A D A K K Q G A V L A W G S F L T L T I N MSCL_HAEIN T P V L G I L T G G I D F K D M K F V L A Q - - - - - - - A Q G D V P A V T L N Y G L F I Q N V I D MSCL_SYNY3 A V L L Q P V L D Q A G V S Q L - - - - - - - - - - - - - - - - - - Q D L P L G L G E L V I A I I N MSCL_Q9RRR4 D P L I R L A T G G H G K V A G T F A V - - - - - - - - - - - - - - N G I T F D W G T F V S T L I N MSCL_CLOPE M P V V G S L V G K V D F S N L Y I N L S G Q Q F N S L Q E A Q A A G A A T I N Y G L F L N N L I N MSCL_MYCTU T P L I N R I - - G V N A Q S D V G I L R I - - - - - - - - - G I G G G Q T I D L N V L L S A A I N MSCL_MYCLE T P L I N R V - - G V N Q Q T N I S P L R I - - - - - - - - - D I G G D Q A I D L N I V L S A A I N MSCL_STAAU M P L I G K I F G S V D F A K E W - - - - - - - - - - - - - - - - - S F W G I K Y G L F I Q S V I D 1msla T P L I N R I - - G V N A Q S D V G I L R I - - - - - - - - - G I G G G Q T I D L N V L L S A A I N Consistency 5 8 5 8 6 4 7 3 5 7 6 6 5 5 4 3 3 2 2 6 3 1 0 0 0 0 0 0 0 1 0 2 1 1 3 3 3 5 7 4 5 6 4 7 8 5 5 6 7 7     . . . . . . . . . 110 . . . . . . . . . 120 . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 MSCL_ECOLI F L I V A F A I F M A I - K L I N K L N R K K E - E P A A - A P A P T - - - - - K E E V L L T E I R MSCL_ERWCA F I I V A F A I F I A I - K L M N K M R C K Q E D T P A A - P P K P S - - - - - A E E K L L A E I R MSCL_PSEFL F V I V A F A I F M G V - K A I N R L K R E E A V A P S E - P P V P S - - - - - A E E T L L T E I R MSCL_CLOHI F L I I S F S I F L F I - R L I N R F K R K E E A V E E A K I P E I S - - - - - R E E E L L G E I R MSCL_BACSU F L I I S F S I F I V I - R T L N G L R R K K E A E E E A A E E A V D - - - - - A Q E E L L K E I R MSCL_Q9RH75 F I I I A F V L F L V I - R A I N T L K R K E E A A P A A - P P K P S - - - - - A E V E L L T E I R MSCL_HAEIN F I I I A F A I F M M I - K V I N K V R K P E E - - - - - - K K T A P - - - - - K A E T L L T E I R MSCL_SYNY3 F L I I A F V I F L I I - K A I E K M Q R K K A V E E E I V A E A Q P D P V L E A Q T N L T D S I N MSCL_Q9RRR4 F L L T A A V L Y F F V V M P M N A A T E R L K R S E K A A E A E P S - - - - - N E E K L L A E I R MSCL_CLOPE F L I I A F S I F I V I - K Q I N K L K N F T K K K E E V K V E A T E - - - - - K D C P Y C Y T K I MSCL_MYCTU F F L I A F A V Y F L V V L P Y N T L R K K G E V - - - - E Q P G D - - - - - - T Q V V L L T E I R MSCL_MYCLE F L L I A L V V Y F L V V L P Y T T I R K H G E V E Q F D T D L I G - - - - - - N Q V V L L A E I R MSCL_STAAU F I I I A F A L F I F V - K I A N T L M K K E E A E E E A V V E - - - - - - - - E N V V L L T E I R 1msla F F L I A F A V Y F L V V L P Y N T L R K K G E V E Q P G D - - - - - - - - - - T Q V V L L T E I R Consistency * 7 8 8 9 8 6 8 8 6 4 9 0 5 4 5 8 5 7 6 6 6 4 7 3 3 4 3 4 1 3 4 3 2 2 0 0 0 0 0 4 6 5 4 9 8 5 8 8 7     . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 . . . . . MSCL_ECOLI D L L K E Q N N R S - - - - - - - - - - - - - - - - - - - - - - - - - MSCL_ERWCA D L L K E Q Q T R Q - - - - - - - - - - - - - - - - - - - - - - - - - MSCL_PSEFL D L L K A Q Q N K S - - - - - - - - - - - - - - - - - - - - - - - - - MSCL_CLOHI D L L K E K N K - - - - - - - - - - - - - - - - - - - - - - - - - - - MSCL_BACSU D L L K Q Q A K S P E - - - - - - - - - - - - - - - - - - - - - - - - MSCL_Q9RH75 D L L K K S C T R T L R P N C - - - - - - - - - - - - - - - - - - - - MSCL_HAEIN D L L K N K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - MSCL_SYNY3 R L I T T L E N Q Q S S S Q - - - - - - - - - - - - - - - - - - - - - MSCL_Q9RRR4 D A V R S R P L - - - - - - - - - - - - - - - - - - - - - - - - - - - MSCL_CLOPE D I K A T R C P H C T S V L E E A T N - - - - - - - - - - - - - - - - MSCL_MYCTU D L L A Q T N G D S P G R H G G R G - - T P S P T D G P R A S T E S Q MSCL_MYCLE D L L A Q S N G A P S G R H V D T A D L T P T P N H E P R A D T - - - MSCL_STAAU D L L R E K K - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1msla - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Consistency 7 7 6 5 4 4 2 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0   (c) IBIVU 2025. 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